Mouse Anti-SERPINA1 Recombinant Antibody (clone 2H2)
CAT#: PABJ-0203
This product is a Mouse antibody that recognizes Human SERPINA1. 2H2 found to react with M A1AT but little against the Z variant.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Mouse Anti-SERPINA1 Recombinant Antibody (PABJ-0203) in SDS-PAGE
SDS-PAGE analysis of PABJ-0203 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 25 kDa and 50 kDa, respectively.
SEC-HPLC
Figure 2 Mouse Anti-SERPINA1 Recombinant Antibody (PABJ-0203) in SEC-HPLC
The purity of PABJ-0203 was 99.1771% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Mouse Anti-SERPINA1 Recombinant Antibody (PABJ-0203) in WB
Western blot analysis of PABJ-0203 was performed with human SERPIN A1 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABJ-0203 and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
DB
Figure 4 Mouse Anti-SERPINA1 Recombinant Antibody (PABJ-0203) in DB
Dot Blot analysis of PABJ-0203 was performed by coating with human SERPIN A1 protein (His tag).
PABJ-0203 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Mouse Anti-SERPINA1 Recombinant Antibody (PABJ-0203) in ELISA
ELISA analysis of PABJ-0203 was performed by coating with human SERPIN A1 protein (His tag). Then blocked with BSA and incubated with PABJ-0203. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/927/1399_A10_5_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/927/2684_B_7_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16648/40122_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/927/2684_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197249-SERPINA1
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SERPINA1
- Species Reactivity
- Human
- Clone
- 2H2
- Applications
- IF, FuncS
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence, Functional Assay.
Target
- Alternative Names
- PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT; alpha-1-antitrypsin; alpha-1 antitrypsin; alpha-1 protease inhibitor; alpha-1-antiproteinase; alpha-1-antitrypsin short transcript variant 1C4; alpha-1-antitrypsin short transcript variant 1C5; epididymis
- Gene ID
- 5265
- UniProt ID
- P01009
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABJ-0203. Click the button above to contact us or submit your feedback about this product.
High Sensitivity in IF
Using the Mouse Anti-SERPINA1 Recombinant Antibody for immunofluorescence has greatly surpassed my expectations. The antibody showed incredible sensitivity, highlighting SERPINA1 localization within my samples with clear and distinct signal differentiation. Its application has tremendously benefited my experimental results, increasing the clarity and precision of SERPINA1-related data, especially in tissues with varied protein expression.
Robust Functionality
The antibody's robust functionality is evident in its application within our lab's functional assays, providing decisive data outcomes when studying SERPIN proteins. Its repeatable success across methodologies underscores its indispensable role in advancing our non-clinical research inquiries.
Useful in Signaling Research
Deploying this antibody has been insightful for examining signaling pathways, notably those influenced by SERPINA1 expression. It offers valuable contributions to research exploring underlying biological mechanisms and reinforcing our hypotheses with tangible data.
Q&As
-
Can this antibody be used in immunofluorescence applications?
A: Yes, the Mouse Anti-SERPINA1 Recombinant Antibody is designed for use in immunofluorescence (IF), providing reliable data on protein localization and expression in cellular contexts. This makes it ideal for visualizing SERPINA1 in various experimental settings.
-
What is the source species of this recombinant antibody?
A: The Mouse Anti-SERPINA1 Recombinant Antibody is sourced from mice, providing a well-characterized system for developing highly specific antibodies used in research applications.
-
What makes this antibody suitable for non-clinical studies?
A: This recombinant antibody is designed with high specificity and minimal cross-reactivity, making it ideal for detailed non-clinical studies where accuracy and reliability are paramount in elucidating SERPINA1 functions.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABJ-0203, RRID: AB_3111616)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 2H2"
- CAT
- Product Name
See other products for "SERPINA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0846MZ | Chicken Anti-alpha-1 Antitrypsin Polyclonal IgY | ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3212 | Hi-Affi™ Recombinant Rabbit Anti-SERPINA1 Monoclonal Antibody (DS3212AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0408-YJ-S(P) | Mouse Anti-SERPINA1 Recombinant Antibody (clone 3H12-2C2); scFv Fragment | ELISA, Inhib | Mouse scFv |
| PABJ-0157-S(P) | Mouse Anti-SERPINA1 Recombinant Antibody (clone 1D9); scFv Fragment | ELISA | Mouse scFv |
| PABJ-0203-S(P) | Mouse Anti-SERPINA1 Recombinant Antibody (clone 2H2); scFv Fragment | IF, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0408-YJ-F(E) | Mouse Anti-SERPINA1 Recombinant Antibody (clone 3H12-2C2); Fab Fragment | ELISA, Inhib | Mouse Fab |
| PABJ-0157-F(E) | Mouse Anti-SERPINA1 Recombinant Antibody (clone 1D9); Fab Fragment | ELISA | Mouse Fab |
| PABJ-0203-F(E) | Mouse Anti-SERPINA1 Recombinant Antibody (clone 2H2); Fab Fragment | IF, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0157 | Mouse Anti-SERPINA1 Recombinant Antibody (clone 1D9) | ELISA | Mouse IgG |
| VS3-CJ102 | Mouse Anti-SERPINA1 Recombinant Antibody (VS3-CJ102) | WB, ELISA | Mouse IgG |
| VS3-XY1410 | Mouse Anti-SERPINA1 Recombinant Antibody (clone 2B12) | ELISA, WB | Mouse IgG1 |
| VS7-0425-WR841 | Mouse Anti-SERPINA1 Recombinant Antibody (clone 7D3A2) | IHC, FC | Mouse IgG1 |
| VS7-0425-WR842 | Mouse Anti-SERPINA1 Recombinant Antibody (clone 8C7A4) | IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1444 | AbPlus™ Anti-Serpina1 Magnetic Beads (VS-0724-YC1444) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6467 | Anti-SERPINA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6468 | Anti-Human SERPINA1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: Neut, ELISA, Inhib, ICC, WB
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, Neut
Application: ELISA, FC, Neut, Inhib
Application: ELISA, Block, WB, FC, IP
Application: ELISA, IHC, IF, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
